화학공학 GRAM staining 그램염색 & observation of bacteria

화학공학 GRAM staining 그램염색 & observation of bacteria

[화학공학] GRAM staining 그램염색 & observation of bacteria.pptx

목차 1.Objective 2.Intro & Background 3.Apparatus & Regents 4.Procedures 5.References 본문 Keeping size and shape of cell Preventing rupture of cells by osmotic pressure Hard because of Peptidoglycan - 20 ~ 80mm : gram-positive - 7 ~ 8 mm : gram-negative 24hours cultured E.Coli in medium Crystal violet Gram iodine 95% ethyl-alcohol Safranin solution (25% safranin in 50% ethyl-alcohol) Wash the slide glass with water quickly and blot dry gently. Smear is now ready to be read with microscope. (Use oil immersion lens.) 참고문헌 http://food.chungkang.ac.kr/ybchoi/lecture/fmexp/fmbexp-10.htm http://www.hitel.net/~U931018/text20ca.htm http://www.hitel.net/~U931018/text20ox.htm http://www.kccm.or.kr/default.asp http://www.labbank.co.kr/Ajoo/product/ion_p1.asp http://en.wikipedia.org/wiki/Gram_staining Microbiology : A laboratory manual (James G.Cappuccino, Natalie Sherman) http://www.enasco.com/product/SB0104M http://safety.skku.edu BROCK BIOLOGY OF MICROORGANISMS 3 EDITION Michael T.Madigan, p78 ~84 Willey 외, 일반미생물학(2008), 라이프사이언스 민철기,일반생물학실험서(1999), 라이프사이언스 키워드 그램염색, 화학공학, GRAM, staining